Enzyme |
PDB code |
Source |
Recognition sequence |
Cut |
Isoschizomers |
Bsp6I |
|
Bacillus sp. RFL6 |
5' GCNGC 3' CGNCG |
5' ---GC NGC--- 3' 3' ---CGN CG--- 5' |
|
Bsp13I |
|
Bacillus sp. 13 |
5' TCCGGA 3' AGGCCT |
5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' |
AccIII, BbvAIII, BlfI, BsiMI, BspMII, Bsu23I, Kpn2I, PinBII |
Bsp19I |
|
Bacillus sp. 19 |
5' CCATGG 3' GGTACC |
5' ---C CATGG--- 3' 3' ---GGTAC C--- 5' |
|
Bsp24I |
|
Bacillus sp. 24 |
5' GACN6TGG 3' CTGN6ACC |
5' ---GACN6TGGN6NNNNNN --- 3' 3' ---CTGN6ACCN6N NNNNN--- 5' |
|
Bsp50I |
|
Bacillus sp. RFL50 |
5' CGCG 3' GCGC |
5' ---CG CG--- 3' 3' ---GC GC--- 5' |
BceBI, Bsh1236I, BstFNI, BstUI, Bsu1532I, FalII, FnuDII, MvnI, ThaI |
Bsp63I |
|
Bacillus sphaericus 63 |
5' CTGCAG 3' GACGTC |
5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' |
AjoI, Asp713I, BloHII, MhaAI, Pfl21I, PstI, SalPI, SflI, Sst12I, YenI |
Bsp67I |
|
Bacillus sphaericus 67 |
5' GATC 3' CTAG |
5' --- GATC--- 3' 3' ---CTAG --- 5' |
AspMDI, Bsp143I, BspJI, BtkII, DpnII, LlaAI, NlaII, SsiBI |
Bsp68I |
|
Bacillus sp. RFL68 |
5' TCGCGA 3' AGCGCT |
5' ---TCG CGA--- 3' 3' ---AGC GCT--- 5' |
NruI |
Bsp98I |
|
Bacillus sp. RFL98 |
5' GGATCC 3' CCTAGG |
5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' |
AccEBI, AliI, BamHI, BnaI, BstI, CelI, OkrAI, NspSAIV, Pfl8I, SurI |
Bsp105I |
|
Bacillus sphaericus 105 |
5' GATC 3' CTAG |
5' --- GATC--- 3' 3' ---CTAG --- 5' |
Bfi57I, Bsp143I, BspJI, BtkII, DpnII, LlaAI, NlaII, SsiBI |
Bsp106I |
|
Bacillus sphaericus 106 |
5' ATCGAT 3' TAGCTA |
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' |
BbvAII, Bci29I, BciBI, BcmI, BspOVII, BstNZ169I, Rme21I |
Bsp119I |
|
Bacillus sp. RFL119 |
5' TTCGAA 3' AAGCTT |
5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' |
AcpI, Bim19I, Bpu14I, BspT104I, Csp45I, FspII, NspV, SspRFI |
Bsp120I |
|
Bacillus sp. RFL120 |
5' GGGCCC 3' CCCGGG |
5' ---G GGCCC--- 3' 3' ---CCCGG G--- 5' |
ApaI, PpeI, PspOMI |
Bsp123I |
|
Bacillus sp. 123 |
5' CGCG 3' GCGC |
5' ---CG CG--- 3' 3' ---GC GC--- 5' |
BceBI, Bsh1236I, BstFNI, BstUI, BtkI, Csp68KVI, FnuDII, MvnI, ThaI |
Bsp143I |
|
Bacillus sp. RFL143 |
5' GATC 3' CTAG |
5' --- GATC--- 3' 3' ---CTAG --- 5' |
Bce243I, Bsp105I, BspFI, BstKTI, CpfI, HacI, NdeII, Sth368I, Sau3AI |
Bsp143II |
|
Bacillus sp. RFL143 |
5' RGCGCY 3' YCGCGR |
5' ---RGCGC Y--- 3' 3' ---Y CGCGR--- 5' |
AccB2I, |
BfoI, |
Bme142I, BstH2I, |
HaeII, LpnI |
|
Bsp211I |
|
Bacillus sphaericus 211 |
5' GGCC 3' CCGG |
5' ---GG CC--- 3' 3' ---CC GG--- 5' |
|
Bsp423I |
|
Bacillus sp. 423 |
5' GCAGC 3' CGTCG |
5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5' |
AlwXI, BbvI, BseKI, BseXI, Bst12I, Bst71I, BstV1I |
Bsp519I |
|
Bacillus sp. 519 |
5' GRGCYC 3' CYCGRG |
5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5' |
|
Bsp1286I |
|
Bacillus sphaericus |
5' GDGCHC 3' CHCGDG |
5' ---GDGCH C--- 3' 3' ---C HCGDG--- 5' |
AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, NspII, SduI |
Bsp1407I |
|
Bacillus stearothermophilus RFL1407 |
5' TGTACA 3' ACATGT |
5' ---T GTACA--- 3' 3' ---ACATG T--- 5' |
AauI, |
BsmRI, |
BsrGI, BstAUI, |
Ssp4800I, SspBI |
|
Bsp1720I |
|
Bacillus sp. 1720 |
5' GCTNAGC 3' CGANTCG |
5' ---GC TNAGC--- 3' 3' ---CGANT CG--- 5' |
|
Bsp1894I |
|
Bacillus sphaericus 1894 |
5' GGNCC 3' CCNGG |
5' ---G GNCC--- 3' 3' ---CCNG G--- 5' |
AspS9I, Bac36I, BavBII, BshKI, BspBII, CcuI, MaeK81II, Pde12I |
Bsp2095I |
|
Bacillus sphaericus 2095 |
5' GATC 3' CTAG |
5' --- GATC--- 3' 3' ---CTAG --- 5' |
Bfi57I, Bsp143I, BspKT6I, BtkII, DpnII, LlaAI, NlaII, SsiBI |
Bsp4009I |
|
Bacillus sp. 4009 |
5' GGATCC 3' CCTAGG |
5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' |
AccEBI, AliI, ApaCI, BamHI, BstI, CelI, OkrAI, Pfl8I, RspLKII, SurI |
BspAI |
|
Bacillus sphaericus JL4B |
5' GATC 3' CTAG |
5' --- GATC--- 3' 3' ---CTAG --- 5' |
Bfi57I, Bsp2095I, BspKT6I, BtkII, DpnII, LlaAI, NlaII, SsiBI |
BspA2I |
|
Bacillus sp. A2 |
5' CCTAGG 3' GGATCC |
5' ---C CTAGG--- 3' 3' ---GGATC C--- 5' |
AspA2I, AvrII, AvrBII, BlnI, XmaJI |
Bsp153AI |
|
Bacillus sp. 153A |
5' CAGCTG 3' GTCGAC |
5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' |
|
BspAAI |
|
Bacillus sp. AA |
5' CTCGAG 3' GAGCTC |
5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' |
AbrI, BluI, BstVI, PanI, SauLPII, SciI, SlaI, StrI, TliI |
BspAAII |
|
Bacillus sp. AA |
5' TCTAGA 3' AGATCT |
5' ---T CTAGA--- 3' 3' ---AGATC T--- 5' |
|
BspAAIII |
|
Bacillus sp. AA |
5' GGATCC 3' CCTAGG |
5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' |
AccEBI, AliI, ApaCI, AsiI, BamHI, Bce751I, Pfl8I, RspLKII, SolI |
BspANI |
|
Bacillus sp. AN |
5' GGCC 3' CCGG |
5' ---GG CC--- 3' 3' ---CC GG--- 5' |
|
BspBI |
|
Bacillus sphaericus JL14 |
5' CTGCAG 3' GACGTC |
5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' |
AjoI, ApiI, Asp713I, BloHII, CfrA4I, Pfl21I, PstI, SalPI, Sst12I, YenI |
BspBII |
|
Bacillus sphaericus JL14 |
5' GGNCC 3' CCNGG |
5' ---G GNCC--- 3' 3' ---CCNG G--- 5' |
AsuI, Bac36I, BavBII, BshKI, BspF4I, CcuI, MaeK81II, PspPI |
BspBRI |
|
Bacillus sp. |
5' GGCC 3' CCGG |
5' ---GG CC--- 3' 3' ---CC GG--- 5' |
|
BspBS31I |
|
Bacillus sp. BS31 |
5' GAAGAC 3' CTTCTG |
5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' |
|
BspCI |
|
Bacillus sp. C1 |
5' CGATCG 3' GCTAGC |
5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5' |
Afa16RI, Afa22MI, EagBI, ErhB9I, NblI, Psu161I, RshI, XorII |
BspCNI |
|
Bacillus sp. 1310 |
5' CTCAG 3' GAGTC |
5' ---CTCAGN6NNN --- 3' 3' ---GAGTCN6N NN--- 5' |
|
BspDI |
|
Bacillus sp. |
5' ATCGAT 3' TAGCTA |
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' |
AagI, BanIII, BavCI, Bsa29I, BseCI, Bsu15I, BsuTUI, ClaI, ZhoI |
BspD6I [7][8] |
2P14 |
Bacillus sp. D6 |
5' GACTC 3' CTGAG |
5' ---GACTCNNNN NN--- 3' 3' ---CTGAGNNNNNN --- 5' |
|
BspEI |
|
Bacillus sp. |
5' TCCGGA 3' AGGCCT |
5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' |
AccIII, BlfI, BsiMI, Bsp13I, BspMII, CauB3I, MroI, PinBII |
BspFI |
|
Bacillus sp. |
5' GATC 3' CTAG |
5' --- GATC--- 3' 3' ---CTAG --- 5' |
Bfi57I, Bsp2095I, BspKT6I, BtkII, DpnII, LlaAI, NlaII, SsiBI |
BspF4I |
|
Bacillus sp. F4 |
5' GGNCC 3' CCNGG |
5' ---G GNCC--- 3' 3' ---CCNG G--- 5' |
AsuI, Bac36I, BavBII, BsiZI, BspBII, CcuI, MaeK81II, PspPI |
BspHI |
|
Bacillus sp. H3 |
5' TCATGA 3' AGTACT |
5' ---T CATGA--- 3' 3' ---AGTAC T--- 5' |
|
BspIS4I |
|
Bacillus sp. IS4 |
5' GAAGAC 3' CTTCTG |
5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' |
|
BspJI |
|
Bacillus sp. |
5' GATC 3' CTAG |
5' --- GATC--- 3' 3' ---CTAG --- 5' |
BfuCI, Bsp2095I, BspKT6I, BtkII, DpnII, LlaAI, NmeCI, SsiAI |
BspJII |
|
Bacillus sp. |
5' ATCGAT 3' TAGCTA |
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' |
Bsa29I, BseCI, Bsp106I, BspDI, BspOVII, BspXI, BspZEI |
BspKI |
|
Bacillus sp. K |
5' GGCC 3' CCGG |
5' ---GG CC--- 3' 3' ---CC GG--- 5' |
|
BspKT5I |
|
Bacillus sp. |
5' CTGAAG 3' GACTTC |
5' ---CTGAAGN12NNNN --- 3' 3' ---GACTTCN12NN NN--- 5' |
|
BspKT6I |
|
Bacillus sp. KT6 |
5' GATC 3' CTAG |
5' ---GAT C--- 3' 3' ---C TAG--- 5' |
BfuCI, Bsp2095I, Bst19II, BstKTI, DpnII, LlaAI, NmeCI, SsiAI |
BspKT8I |
|
Bacillus sp. KT8 |
5' AAGCTT 3' TTCGAA |
5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5' |
|
BspLI |
|
Bacillus sp. RJ3-212 |
5' GGNNCC 3' CCNNGG |
5' ---GGN NCC--- 3' 3' ---CCN NGG--- 5' |
AspNI, BscBI, |
BmiI, |
NlaIV, |
PspN4I |
|
BspLAI |
|
Bacillus sp. LA |
5' GCGC 3' CGCG |
5' ---GCG C--- 3' 3' ---C GCG--- 5' |
AspLEI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI, SciNI |
BspLAII |
|
Bacillus sp. LA |
5' TTCGAA 3' AAGCTT |
5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' |
|
BspLAIII |
|
Bacillus sp. LA |
5' AAGCTT 3' TTCGAA |
5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5' |
|
BspLS2I |
|
Bacillus sp. LS2 |
5' GDGCHC 3' CHCGDG |
5' ---GDGCH C--- 3' 3' ---C HCGDG--- 5' |
AocII, BmyI, BsoCI, BspLS2I, MhlI, NspII, SduI |
BspLU4I |
|
Bacillus sp. LU4 |
5' CYCGRG 3' GRGCYC |
5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' |
Ama87I, AvaI, Bse15I, BsoBI, BstSI, NspIII, NspSAI, OfoI |
BspLU11I |
|
Bacillus sp. LU11 |
5' ACATGT 3' TGTACA |
5' ---A CATGT--- 3' 3' ---TGTAC A--- 5' |
|
BspLU11III |
|
Bacillus sp. LU11 |
5' GGGAC 3' CCCTG |
5' ---GGGACN8NN NNNN--- 3' 3' ---CCCTGN8NNNNNN --- 5' |
|
BspMI |
|
Bacillus sp. M |
5' ACCTGC 3' TGGACG |
5' ---ACCTGCNNNN NNNN--- 3' 3' ---TGGACGNNNNNNNN --- 5' |
Acc36I, BfuAI, BveI |
BspMII |
|
Bacillus sp. M |
5' TCCGGA 3' AGGCCT |
5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' |
AccIII, BbvAIII, BlfI, BsiMI, BspEI, Bsu23I, CauB3I, MroI |
BspM39I |
|
Bacillus sp. M39 |
5' CAGCTG 3' GTCGAC |
5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' |
|
BspM90I |
|
Bacillus sp. M90 |
5' GTATAC 3' CATATG |
5' ---GTA TAC--- 3' 3' ---CAT ATG--- 5' |
|
BspMAI |
|
Bacillus stearothermophilus MA |
5' CTGCAG 3' GACGTC |
5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' |
AjoI, Asp713I, BloHII, PaePI, Psp23I, PstI, SalPI, Sst12I, YenI |
BspMKI |
|
Bacillus sp. MKI |
5' GTCGAC 3' CAGCTG |
5' ---G TCGAC--- 3' 3' ---CAGCT G--- 5' |
|
BspNI |
|
Bacillus sp. N |
5' CCWGG 3' GGWCC |
5' ---CC WGG--- 3' 3' ---GGW CC--- 5' |
AglI, BseBI, Bse17I, Bst2I, CbrI, CthII, EcoRII, MvaI, SspAI, TaqXI |
BspO4I |
|
Bacillus sp. O-4 |
5' CAGCTG 3' GTCGAC |
5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' |
|
BspOVI |
|
Bacillus sp. OV |
5' GACN5GTC 3' CTGN5CAG |
5' ---GACNNN NNGTC--- 3' 3' ---CTGNN NNNCAG--- 5' |
AhdI, AspEI, |
BmeRI, |
DriI, |
Eam1105I, EclHKI, NruGI |
|
BspOVII |
|
Bacillus sp. OV |
5' ATCGAT 3' TAGCTA |
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' |
BanIII, BavCI, BciBI, BcmI, Bli86I, BliRI, BspJII, BstNZ169I, SpmI |
BspPI |
|
Bacillus sp. d 1-34 |
5' GGATC 3' CCTAG |
5' ---GGATCNNNN N--- 3' 3' ---CCTAGNNNNN --- 5' |
AclWI, AlwI, BinI, |
BsrWI, |
BstH9I, |
Bst31TI, Bth617I, |
EacI |
|
BspRI |
|
Bacillus sphaericus R |
5' GGCC 3' CCGG |
5' ---GG CC--- 3' 3' ---CC GG--- 5' |
|
BspR7I |
|
Bacillus sp. R7 |
5' CCTNAGG 3' GGANTCC |
5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5' |
AocI, AxyI, Bse21I, Bsu36I, Eco81I, MstII, SauI, SshAI |
BspST5I |
|
Bacillus sp. ST5 |
5' GCATC 3' CGTAG |
5' ---GCATCN4N NNNN--- 3' 3' ---CGTAGN4NNNNN --- 5' |
|
BspTI |
|
Bacillus sp. RFL1265 |
5' CTTAAG 3' GAATTC |
5' ---C TTAAG--- 3' 3' ---GAATT C--- 5' |
AflII |
BspT104I |
|
Bacillus sp. T104 |
5' TTCGAA 3' AAGCTT |
5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' |
AcpI, Bim19I, Bpu14I, BstBI, Csp45I, LspI, NspV, SspRFI |
BspT107I |
|
Bacillus sp. T107 |
5' GGYRCC 3' CCRYGG |
5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5' |
AccB1I, BanI, BbvBI, Eco64I, HgiCI, HgiHI, MspB4I, PfaAI |
BspTNI |
|
Bacillus sp. TN |
5' GGTCTC 3' CCAGAG |
5' ---GGTCTCN NNNN--- 3' 3' ---CCAGAGNNNNN --- 5' |
|
BspTS514I |
|
Bacillus sp. TS514 |
5' GAAGAC 3' CTTCTG |
5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' |
|
BspWI |
|
Bacillus sp. 3456 |
5' GCN7GC 3' CGN7CG |
5' ---GCNNNNN NNGC--- 3' 3' ---CGNN NNNNNCG--- 5' |
|
BspXI |
|
Bacillus sphaericus X |
5' ATCGAT 3' TAGCTA |
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' |
BliAI, BliRI, BscI, Bst28I, Bsu15I, LcaI, PgaI, Ssp27144I, ZhoI |
BspXII |
|
Bacillus sphaericus X |
5' TGATCA 3' ACTAGT |
5' ---T GATCA--- 3' 3' ---ACTAG T--- 5' |
AbaI, BclI, BsiQI, |
BstT7I, FbaI, Ksp22I, |
ParI |
|
BspZEI |
|
Bacillus sp. ZE |
5' ATCGAT 3' TAGCTA |
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' |
Bsa29I, BseCI, BspDI, BspXI, Bst28I, BstNZ169I, BsuTUI, LplI |