List of restriction enzyme cutting sites: T–Z

Main articles: List of restriction enzyme cutting sites and Restriction enzyme

Legend of nucleobases
Code	Nucleotide represented
A	Adenine (A)
C	Cytosine (C)
G	Guanine (G)
T	Thymine (T)
N	A, C, G or T
M	A or C
R	A or G
W	A or T
Y	C or T
S	C or G
K	G or T
H	A, C or T
B	C, G or T
V	A, C or G
D	A, G or T

This article contains a list of the most studied restriction enzymes whose names start with T to Z inclusive. It contains approximately 70 enzymes.

The following information is given:

Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature^[1]^[2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action^[3]^[4].
Source: Organism that naturally produces the enzyme.
Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site^[5]^[6].
Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:

as in this not listed enzyme:

EcoR70I

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Ba-Bc
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See also

Restriction enzymes

T

Current list/Article:

Enzyme	PDB code	Source	Recognition sequence	Cut	Isoschizomers
TaaI		Thermus aquaticus Vn 4-311	`5' ACNGT` `3' TGNCA`	`5' ---ACN GT--- 3'` `3' ---TG NCA--- 5'`
TaiI		Thermus aquaticus cs1-331	`5' ACGT` `3' TGCA`	`5' ---ACGT --- 3'` `3' --- TGCA--- 5'`
TaqI		Thermus aquaticus	`5' TCGA` `3' AGCT`	`5' ---T CGA--- 3'` `3' ---AGC T--- 5'`
TaqII		Thermus aquaticus YTI	`5' GACCGACACCCA` `3' CTGGCTGTGGGT`	`5' ---GACCGACACCCAN₈NNN --- 3'` `3' ---CTGGCTGTGGGTN₈N NN--- 5'`
Taq52I		Thermus aquaticus YS52	`5' GCWGC` `3' CGWCG`	`5' ---G CWGC--- 3'` `3' ---CGWC G--- 5'`	AceI, ApeKI, SuiI, TseI
TaqXI		Thermus aquaticus	`5' CCWGG` `3' GGWCC`	`5' ---CC WGG--- 3'` `3' ---GGW CC--- 5'`	ApaORI, BseBI, BsiLI, BstNI, BstOI, Bst2UI, MvaI, SleI, SspAI
TasI		Thermus aquaticus Vn 4-211	`5' AATT` `3' TTAA`	`5' --- AATT--- 3'` `3' ---TTAA --- 5'`
TatI		Thermus aquaticus CBA1-331	`5' WGTACW` `3' WCATGW`	`5' ---W GTACW--- 3'` `3' ---WCATG W--- 5'`
TauI		Thermus aquaticus	`5' GCSGC` `3' CGSCG`	`5' ---GCSG C--- 3'` `3' ---C GSCG--- 5'`
TelI		Tolypothrix elabens	`5' GACNNNGTC` `3' CTGNNNCAG`	`5' ---GACN NNGTC--- 3'` `3' ---CTGNN NCAG--- 5'`	AspI, AtsI, PflFI, PsyI, Tth111I
TfiI		Thermus filiformis	`5' GAWTC` `3' CTWAG`	`5' ---G AWTC--- 3'` `3' ---CTWA G--- 5'`
ThaI		Thermoplasma acidophilum	`5' CGCG` `3' GCGC`	`5' ---CG CG--- 3'` `3' ---GC GC--- 5'`	Bpu95I, Bsh1236I, Bsp50I, BstFNI, BstUI, Csp68KVI, FalII, FnuDII
TliI		Thermococcus litoralis	`5' CTCGAG` `3' GAGCTC`	`5' ---C TCGAG--- 3'` `3' ---GAGCT C--- 5'`	AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, StrI, XhoI
Tru1I		Thermus ruber RFL1	`5' TTAA` `3' AATT`	`5' ---T TAA--- 3'` `3' ---AAT T--- 5'`
Tru9I		Thermus ruber 9	`5' TTAA` `3' AATT`	`5' ---T TAA--- 3'` `3' ---AAT T--- 5'`
Tru201I		Thermus ruber 201	`5' RGATCY` `3' YCTAGR`	`5' ---R GATCY--- 3'` `3' ---YCTAG R--- 5'`
TscI		Thermus sp. 491A	`5' ACGT` `3' TGCA`	`5' ---ACGT --- 3'` `3' --- TGCA--- 5'`
TseI		Thermus sp. 93170	`5' GCWGC` `3' CGWCG`	`5' ---G CWGC--- 3'` `3' ---CGWC G--- 5'`	AceI, ApeKI, SuiI, Taq52I
Tsp1I		Thermus sp. 1	`5' ACTGG` `3' TGACC`	`5' ---ACTGGN --- 3'` `3' ---TGAC CN--- 5'`
Tsp32I		Thermus sp. 32	`5' TCGA` `3' AGCT`	`5' ---T CGA--- 3'` `3' ---AGC T--- 5'`
Tsp32II		Thermus sp. 32	`5' TCGA` `3' AGCT`	`5' ---T CGA--- 3'` `3' ---AGC T--- 5'`
Tsp45I		Thermus sp. YS45	`5' GTSAC` `3' CASTG`	`5' --- GTSAC--- 3'` `3' ---CASTG --- 5'`
Tsp49I		Thermus sp.	`5' ACGT` `3' TGCA`	`5' ---ACGT --- 3'` `3' --- TGCA--- 5'`
Tsp509I		Thermus sp.	`5' AATT` `3' TTAA`	`5' --- AATT--- 3'` `3' ---TTAA --- 5'`
TspBI		Thermophilic sp.	`5' CCRYGG` `3' GGYRCC`	`5' ---C CRYGG--- 3'` `3' ---GGYRC C--- 5'`
Tsp4CI		Thermus sp. 4C	`5' ACNGT` `3' TGNCA`	`5' ---ACN GT--- 3'` `3' ---TG NCA--- 5'`
TspDTI		Thermus sp. DT	`5' ATGAA` `3' TACTT`	`5' ---ATGAAN₈NNN --- 3'` `3' ---TACTTN₈N NN--- 5'`	— None on May 2010 —
TspEI		Thermus sp. 1E	`5' AATT` `3' TTAA`	`5' --- AATT--- 3'` `3' ---TTAA --- 5'`
Tsp8EI		Thermus sp. 8E	`5' GCCN₅GGC` `3' CGGN₅CCG`	`5' ---GCCNNNN NGGC--- 3'` `3' ---CGGN NNNNCCG--- 5'`
TspGWI		Thermus sp. GW	`5' ACGGA` `3' TGCCT`	`5' ---ACGGAN₈NNN --- 3'` `3' ---TGCCTN₈N NN--- 5'`
TspMI = UthSI		Unidentified thermophile	`5' CCCGGG` `3' GGGCCC`	`5' ---C CCGGG--- 3'` `3' ---GGGCC C--- 5'`	AhyI, Cfr9I, EaeAI, EclRI, PaeBI, PspAI, SmaI, XcyI, XmaI, XmaCI
TspRI		Thermus sp. R	`5' CASTGNN` `3' GTSAC`	`5' ---CASTGNN --- 3'` `3' --- GTSACNN--- 5'`
Tth111I		Thermus thermophilus 111	`5' GACNNNGTC` `3' CTGNNNCAG`	`5' ---GACN NNGTC--- 3'` `3' ---CTGNN NCAG--- 5'`	AspI, AtsI, PflFI, PsyI, TelI
Tth111II		Thermus thermophilus 111	`5' CAARCA` `3' GTTYGT`	`5' ---CAARCAN₈NNN --- 3'` `3' ---GTTYGTN₈N NN--- 5'`
TthHB8I		Thermus thermophilus HB8	`5' TCGA` `3' AGCT`	`5' ---T CGA--- 3'` `3' ---AGC T--- 5'`

This list is incomplete; you can help by expanding it.

U

Current list/Article:

Enzyme	PDB code	Source	Recognition sequence	Cut	Isoschizomers
Uba4009I		Unidentified bacterium A	`5' GGATCC` `3' CCTAGG`	`5' ---G GATCC--- 3'` `3' ---CCTAG G--- 5'`	AccEBI, AliI, ApaCI, AsiI, BamHI, BnaI, BspAAIII, RspLKII, SolI
Uba153AI		Unidentified bacterium 153A	`5' CAGCTG` `3' GTCGAC`	`5' ---CAG CTG--- 3'` `3' ---GTC GAC--- 5'`
UbaM39I		Unidentified bacterium M39	`5' CAGCTG` `3' GTCGAC`	`5' ---CAG CTG--- 3'` `3' ---GTC GAC--- 5'`
UnbI		Unidentified bacterium #8	`5' GGNCC` `3' CCNGG`	`5' --- GGNCC--- 3'` `3' ---CCNGG --- 5'`	AvcI, BavAII, Bce22I, Bsp1894I, Bsu54I, FmuI, NspIV, UnbI
Uur960I		Ureaplasma urealyticum 960	`5' GCNGC` `3' CGNCG`	`5' ---GC NGC--- 3'` `3' ---CGN CG--- 5'`

This list is incomplete; you can help by expanding it.

V

Current list/Article:

Enzyme	PDB code	Source	Recognition sequence	Cut	Isoschizomers
Van91I		Vibrio anguillarum RFL91	`5' CCAN₅TGG` `3' GGTN₅ACC`	`5' ---CCANNNN NTGG--- 3'` `3' ---GGTN NNNNACC--- 5'`	AccB7I, AcpII, Asp10HII, BasI, Esp1396I, PflBI, PflMI
Vha464I		Vibrio harveyi 464	`5' CTTAAG` `3' GAATTC`	`5' ---C TTAAG--- 3'` `3' ---GAATT C--- 5'`
VneI		Vibrio nereis 18	`5' GTGCAC` `3' CACGTG`	`5' ---G TGCAC--- 3'` `3' ---CACGT G--- 5'`	Alw44I, ApaLI, SnoI
VpaK32I		Vibrio parahaemolyticus 4387-61	`5' GCTCTTC` `3' CGAGAAG`	`5' ---GCTCTTCN NNN--- 3'` `3' ---CGAGAAGNNNN --- 5'`
VpaK11AI		Vibrio parahaemolyticus 1743	`5' GGWCC` `3' CCWGG`	`5' --- GGWCC--- 3'` `3' ---CCWGG --- 5'`	Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, SinI, VpaK11BI
VpaK11BI		Vibrio parahaemolyticus 1743-1	`5' GGWCC` `3' CCWGG`	`5' ---G GWCC--- 3'` `3' ---CCWG G--- 5'`	BsrAI, CauI, EagMI, FdiI, HgiBI, HgiJI, SinI, VpaK11AI
VspI		Vibrio sp. 343	`5' ATTAAT` `3' TAATTA`	`5' ---AT TAAT--- 3'` `3' ---TAAT TA--- 5'`	AseI, AsnI, BpoAI, PshBI, Sru4DI

This list is incomplete; you can help by expanding it.

X

Current list/Article:

Enzyme PDB code Source Recognition sequence Cut Isoschizomers

XagI Xanthobacter agilis Vs 18-132 5' CCTN₅AGG
3' GGAN₅TCC 5' ---CCTNN NNNAGG--- 3'
3' ---GGANNN NNTCC--- 5'

XapI

Xanthomonas ampelina Slo 51-021

5' RAATTY
3' YTTAAR

5' ---R AATTY--- 3'
3' ---YTTAA R--- 5'

AcsI, ApoI,

CfaI,

FsiI

XbaI ^[7] Xanthomonas badrii 5' TCTAGA
3' AGATCT 5' ---T CTAGA--- 3'
3' ---AGATC T--- 5'

XcaI Xanthomonas campestris 5' GTATAC
3' CATATG 5' ---GTA TAC--- 3'
3' ---CAT ATG--- 5'

XceI Xanthomonas campestris Ast 40-024 5' RCATGY
3' YGTACR 5' ---RCATG Y--- 3'
3' ---Y GTACR--- 5'

XciI Xanthomonas citri 5' GTCGAC
3' CAGCTG 5' ---G TCGAC--- 3'
3' ---CAGCT G--- 5'

XcmI Xanthomonas campestris 5' CCAN₉TGG
3' GGTN₉ACC 5' ---CCANNNNN NNNNTGG--- 3'
3' ---GGTNNNN NNNNNACC--- 5'

XcyI Xanthomonas cyanopsidis 13D5 5' CCCGGG
3' GGGCCC 5' ---C CCGGG--- 3'
3' ---GGGCC C--- 5' CfrJ4I, EaeAI, EclRI, Pac25I, PspAI, TspMI, XmaI, XmaCI

XhoI Xanthomonas holcicola 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' AbrI, BluI, BssHI, PanI, Sau3239I, Sfr274I, TliI, XpaI

XhoII Xanthomonas holcicola 5' RGATCY
3' YCTAGR 5' ---R GATCY--- 3'
3' ---YCTAG R--- 5'

XmaI Xanthomonas malvacearum 5' CCCGGG
3' GGGCCC 5' ---C CCGGG--- 3'
3' ---GGGCC C--- 5' AhyI, Cfr9I, EaeAI, EclRI, PaeBI, PspAI, TspMI, XcyI, XmaCI

XmaIII Xanthomonas malvacearum 5' CGGCCG
3' GCCGGC 5' ---C GGCCG--- 3'
3' ---GCCGG C--- 5' AaaI, BseX3I, BstZI, EagI, EclXI, Eco52I, SenPT16I

XmaCI Xanthomonas malvacearum C 5' CCCGGG
3' GGGCCC 5' ---C CCGGG--- 3'
3' ---GGGCC C--- 5' AhyI, Cfr9I, EaeAI, EclRI, PaeBI, Pac25I, PspAI, TspMI, XcyI, XmaI

XmaJI Xanthomonas maltophilia Jo 85-025 5' CCTAGG
3' GGATCC 5' ---C CTAGG--- 3'
3' ---GGATC C--- 5' AspA2I, AvrII, AvrBII, BlnI, BspA2I

XmiI Xanthomonas maltophilia Jo 21-021 5' GTMKAC
3' CAKMTG 5' ---GT MKAC--- 3'
3' ---CAKM TG--- 5' AccI, FblI

XmnI Xanthomonas manihotis 7AS1 5' GAAN₄TTC
3' CTTN₄AAG 5' ---GAANN NNTTC--- 3'
3' ---CTTNN NNAAG--- 5' Asp700I, BbvAI, MroXI, PdmI

XorII Xanthomonas oryzae 5' CGATCG
3' GCTAGC 5' ---CGAT CG--- 3'
3' ---GC TAGC--- 5' Afa16RI, BspCI, EagBI, ErhB9I, MvrI, Ple19I, PvuI, RshI

XpaI Xanthomonas papavericola 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' BssHI, MavI, PanI, SauLPII, Sbi68I, Sol10179I, StrI, XhoI

XspI Xanthomonas sp. YK1 5' CTAG
3' GATC 5' ---C TAG--- 3'
3' ---GAT C--- 5'

This list is incomplete; you can help by expanding it.

Y

Current list/Article:

Enzyme	PDB code	Source	Recognition sequence	Cut	Isoschizomers
YenI		Yersinia enterocolitica 08 A2635	`5' CTGCAG` `3' GACGTC`	`5' ---CTGCA G--- 3'` `3' ---G ACGTC--- 5'`	Asp713I, BsuBI, CfrA4I, Ecl37kI, Psp23I, PstI, SalPI, SflI, Sst12I

This list is incomplete; you can help by expanding it.

Z

Current list/Article:

Enzyme PDB code Source Recognition sequence Cut Isoschizomers

ZanI Zymomonas anaerobia 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' ApaORI, BseBI, BspNI, BstNI, Bst2UI, CthII, EcoRII, MvaI, SspAI

ZhoI Zymomonas holcicola 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' BanIII, BbvAII, BscI, BspJI, ClaI, LcaI, PgaI, SpmI, Ssp27144I

ZraI Zoogloea ramigera 11 5' GACGTC
3' CTGCAG 5' ---GAC GTC--- 3'
3' ---CTG CAG--- 5' AatII, Ssp5230I

ZrmI

Zoogloea ramigera SCA

5' AGTACT
3' TCATGA

5' ---AGT ACT--- 3'
3' ---TCA TGA--- 5'

Acc113I, AssI,	BmcAI, Bpa34I,
DpaI, Eco255I, RflFII, ScaI

Zsp2I Zoogloea sp. 2 5' ATGCAT
3' TACGTA 5' ---ATGCA T--- 3'
3' ---T ACGTA--- 5' BfrBI, Csp68KIII, EcoT22I, NsiI, PinBI, Ppu10I, SepI, SspD5II

This list is incomplete; you can help by expanding it.

Current list/Article:

Notes

↑ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
↑ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790. PMID 12654995.
↑ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
↑ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
↑ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1-2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
↑ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.
↑ Monty Krieger; Matthew P Scott; Matsudaira, Paul T.; Lodish, Harvey F.; Darnell, James E.; Lawrence Zipursky; Kaiser, Chris; Arnold Berk (2004). Molecular Cell Biology (5th ed.). New York: W.H. Freeman and Company. ISBN 0-7167-4366-3.

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