Bisbenzimide
Names | |
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IUPAC name
2’-(4-Ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-1H,3’H-2,5’-bibenzimidazole | |
Other names
Hoechst 33342 | |
Identifiers | |
23491-52-3 | |
3D model (Jmol) | Interactive image |
ChemSpider | 1420 |
ECHA InfoCard | 100.041.523 |
PubChem | 1464 |
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Properties | |
C27H28N6O | |
Molar mass | 452.56 g·mol−1 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
Infobox references | |
Bisbenzimide is an organic compound used as a fluorescent stain for DNA in molecular biology applications. Several bisbenzimides are used and are collectively called Hoechst stains.
Application
Bisbenzimides tend to bind to A-T rich regions of DNA and can decrease its density. Bisbenzimide mixed with DNA samples can then be used to separate DNA according to their AT percentage using a cesium chloride (CsCl) gradient centrifugation.
References
- Sigma Aldrich Product Information Page
- Fluorescence Spectra: http://www.fluorophores.tugraz.at/substance/463
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