Glycoside hydrolase family 48
| Glyco_hydro_48 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 x-tal structure of the mutant e44q of the cellulase cel48f in complex with a thiooligosaccharide | |||||||||
| Identifiers | |||||||||
| Symbol | Glyco_hydro_48 | ||||||||
| Pfam | PF02011 | ||||||||
| Pfam clan | CL0059 | ||||||||
| InterPro | IPR000556 | ||||||||
| SCOP | 1fce | ||||||||
| SUPERFAMILY | 1fce | ||||||||
| CAZy | GH48 | ||||||||
| |||||||||
In molecular biology, glycoside hydrolase family 48 is a family of glycoside hydrolases.
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site,[4] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[5]
Glycoside hydrolase family 48 CAZY GH_48 comprises enzymes with several known activities; endoglucanase (EC 3.2.1.4); cellobiohydrolase (EC 3.2.1.91).
An example of an enzyme containing a domain belonging to this family is one of the cellulases (celA) from the genome of the thermophilic anaerobic bacterium Caldocellum saccharolyticum. The celA gene product is a polypeptide of 1751 amino acids; this has a multidomain structure comprising two catalytic domains and two cellulose-binding domains, linked by Pro-Thr-rich regions. The N-terminal domain encodes an endoglucanase activity on carboxymethylcellulose, consistent with its similarity to several endo-1, 4-beta-D-glucanase sequences, and is a member of the glycoside hydrolase family 9. The C-terminal domain belongs to this family shows similarity to a cellulase from Clostridium thermocellum (CelS), which acts synergistically with a second component to hydrolyse crystalline cellulose.[6]
References
- ↑ Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proc. Natl. Acad. Sci. U.S.A. 92 (15): 7090–7094. doi:10.1073/pnas.92.15.7090. PMC 41477
. PMID 7624375. - ↑ Henrissat B, Davies G (1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–859. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
- ↑ Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999.
- ↑ Henrissat, B. and Coutinho P.M. "Carbohydrate-Active Enzymes server". 1999.
- ↑ CAZypedia, an online encyclopedia of carbohydrate-active enzymes.
- ↑ Saul DJ, Bergquist PL, Te o VS (1995). "celA, another gene coding for a multidomain cellulase from the extreme thermophile Caldocellum saccharolyticum". Appl. Microbiol. Biotechnol. 43 (2): 291–296. doi:10.1007/BF00172827. PMID 7612247.
This article incorporates text from the public domain Pfam and InterPro IPR000556