Fura-2-acetoxymethyl ester
Names | |
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IUPAC name
Acetoxymethyl 2-[5-[bis[(acetoxymethoxy-oxo-
methyl)methyl]amino]-4-[2-[2-[bis[(acetoxymethoxy-oxo- methyl)methyl]amino]-5-methyl-phenoxy]ethoxy]benzofuran- 2-yl]oxazole-5-carboxylate | |
Other names
Fura-2AM | |
Identifiers | |
108964-32-5 | |
3D model (Jmol) | Interactive image |
ChemSpider | 2610364 |
PubChem | 3364574 |
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Properties | |
C44H47N3O24 | |
Molar mass | 1001.85 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
verify (what is ?) | |
Infobox references | |
Fura-2-acetoxymethyl ester, often abbreviated Fura-2AM, is a membrane-permeable derivative of the ratiometric calcium indicator Fura-2 used in biochemistry to measure cellular calcium concentrations by fluorescence. When added to cells, Fura-2AM crosses cell membranes and once inside the cell, the acetoxymethyl groups are removed by cellular esterases. Removal of the acetoxymethyl esters regenerates "Fura-2", the pentacarboxylate calcium indicator. Measurement of Ca2+-induced fluorescence at both 340 nm and 380 nm allows for calculation of calcium concentrations based 340/380 ratios. The use of the ratio automatically cancels out certain variables such as local differences in fura-2 concentration or cell thickness that would otherwise lead to artifacts when attempting to image calcium concentrations in cells.