dnaD

Primosomal protein DnaD
Identifiers
Symbol	dnaI

DnaD is a 232 amino acid long protein that is part of the primosome involved in prokaryotic DNA replication.^[1] In Bacillus subtilis, genetic analysis has revealed three primosomal proteins, DnaB, DnaD, and DnaI, that have no obvious homologues in E. coli. They are involved in primosome function both at arrested replication forks and at the chromosomal origin.^[2]

DnaB and DnaD proteins are both multimeric and bind individually to DNA. DnaD induces DnaB to bind. DnaD alone and the DnaD/DnaB complex then interact with PriA of Bacillus subtilis at several DNA sites. This suggests that the nucleoprotein assembly is sequential in the PriA, DnaD, DnaB order.^[3]

References

↑ Bruand, C.; Sorokin, A.; Serror, P.; Ehrlich, S. D. (1995). "Nucleotide sequence of the Bacillus subtilis dnaD gene". Microbiology. 141 (2): 321–2. doi:10.1099/13500872-141-2-321. PMID 7704260.
↑ Ishigo-oka, D.; Ogasawara, N.; Moriya, S. (2001). "DnaD Protein of Bacillus subtilis Interacts with DnaA, the Initiator Protein of Replication". Journal of Bacteriology. 183 (6): 2148–50. doi:10.1128/JB.183.6.2148-2150.2001. PMC 95117. PMID 11222620.
↑ Marsin, S.; McGovern, S.; Ehrlich, S. D.; Bruand, C.; Polard, P. (2001). "Early Steps of Bacillus subtilis Primosome Assembly". Journal of Biological Chemistry. 276 (49): 45818–25. doi:10.1074/jbc.M101996200. PMID 11585815.

DNA replication (comparing Prokaryotic to Eukaryotic)

Initiation

Prokaryotic (initiation)	Pre-replication complex dnaC Cdc6 Helicase dnaA dnaB T7 Primase dnaG

Eukaryotic (preparation in G1 phase)	Pre-replication complex Origin recognition complex ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 Cdc6 Cdt1 Minichromosome maintenance MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 Licensing factor Autonomously replicating sequence Single-strand binding protein SSBP2 SSBP3 SSBP4 RNase H RNASEH1 RNASEH2A Helicase: HFM1 Primase: PRIM1 PRIM2

Both	Origin of replication/Ori/Replicon Replication fork Lagging and leading strands Okazaki fragments Primer

Replication

Prokaryotic (elongation)	DNA polymerase III holoenzyme dnaC dnaE dnaH dnaN dnaQ dnaT dnaX holA holB holC holD holE Replisome DNA ligase DNA clamp Topoisomerase DNA gyrase Prokaryotic DNA polymerase: DNA polymerase I Klenow fragment

Eukaryotic (synthesis in S phase)	Replication factor C RFC1 Flap endonuclease FEN1 Topoisomerase Replication protein A RPA1 Eukaryotic DNA polymerase: alpha POLA1 POLA2 PRIM1 PRIM2 delta POLD1 POLD2 POLD3 POLD4 epsilon POLE POLE2 POLE3 POLE4 DNA clamp PCNA Control of chromosome duplication

Both	Movement: Processivity DNA ligase

Termination

Telomere: Telomerase
- TERT
- TERC
- DKC1

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