Secretion assay

Secretion assay is a process used in cell biology to identify cells that are secreting a particular protein (usually a cytokine). It was first developed by Manz et al. in 1995.

Usually, a cell that is secreting the protein of interest is isolated using an antibody-antibody complex that coats the cell and is able to "catch" the secreted molecules. The cell is then detected by another fluorochrome-labelled antibody, and is subsequently extracted using a process called fluorescent-activated cell sorting (FACS). The FACS method is broadly similar to the enzyme-linked immunosorbent assay (ELISA) antibody format, except that the encapsulated cells remain intact. This is advantageous as the cells are still living after the extraction has taken place.

Further advances now mean that it is possible to extract the secreting cells using a magnetic-based separation system or using a flow cytometer.

A number of commercial applications exist for secretion assay. One such example is the Gel Microdrop (GMD) technology, developed by One Cell Systems. One Cell asserts that GMD typically recovers a higher number of viable secreting cells than other methods, whilst ignoring any cells which are not secreting the desired protein.

External links

One Cell Systems - Gel Microdrop (GMD) Secretion Assay technology.
Turcanu V, Williams NA Cell identification and isolation on the basis of cytokine secretion: a novel tool for investigating immune responses Nat Med. 2001 Mar;7(3):373-6

References

Manz, Rudolf; et al. (March 1995). "Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix". Proceedings of the National Academy of Sciences. 92: 1921–25. doi:10.1073/pnas.92.6.1921. PMC 42394.

Proteins: key methods of study

Experimental	Protein purification Green fluorescent protein Western blot Protein immunostaining Protein sequencing Gel electrophoresis/Protein electrophoresis Protein immunoprecipitation Peptide mass fingerprinting/Protein mass spectrometry Dual-polarization interferometry Microscale thermophoresis Chromatin immunoprecipitation Surface plasmon resonance Isothermal titration calorimetry X-ray crystallography Protein NMR Cryo-electron microscopy Freeze-fracture electron microscopy

Bioinformatics	Protein structure prediction Protein–protein docking Protein structural alignment Protein ontology Protein–protein interaction prediction

Assay	Enzyme assay Protein assay Secretion assay

Display techniques	Bacterial display mRNA display Phage display Ribosome display Yeast display

Super-resolution microscopy	Photoactivated localization microscopy Vertico SMI

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