Animal testing on rodents

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Rodents are commonly used in animal testing, particularly mice and rats, but also guinea pigs, hamsters, gerbils and others.

Statistics

In the UK in 2014, there were 3.21 million procedures on rodents (83% of total procedures that year). The most common species used were mice (2.92 million prcedures, or 76% of total) and rats (254,000, or 7%). Other rodents species included guinea pigs (27,027 / 0.7%), hamsters (2,785 / 0.1%) and gerbils (401 / 0.01%).^[1]

In the U.S., the numbers of rats and mice used are not reported, but estimates range from around 11 million^[2] to approximately 100 million.^[3] In 2000, the Federal Research Division, Library of Congress, published the results of an analysis of its Rats/Mice/and Birds Database: Researchers, Breeders, Transporters, and Exhibitors.

Over 2,000 research organizations are listed in the database, of which approximately 500 were researched and of these, 100 were contacted directly by FRD staff. These organizations include hospitals, government organizations, private companies (pharmaceutical companies, etc.), universities/colleges, a few secondary schools, and research institutes. Of these 2,000, approximately 960 are regulated by USDA; 349 by NIH; and 560 accredited by AALAC. Approximately 50 percent of the organizations contacted revealed a specific or approximated number of animals in their laboratories. The total number of animals for those organizations is: 250,000–1,000,000 rats; 400,000–2,000,000 mice; and 130,000–900,000 birds.

Rodent types

Mice

Mice are the most commonly used vertebrate species, popular because of their availability, size, low cost, ease of handling, and fast reproduction rate.^[4]

They are widely considered to be the prime model of inherited human disease and share 99% of their genes with humans.^[5] With the advent of genetic engineering technology, genetically modified mice can be generated to order and can cost hundreds of dollars each.^[6]

Transgenic animal production consists of injecting each construct into 300–350 eggs, typically representing three days' work. Twenty to fifty mice will normally be born from this number of injected eggs. These animals are screened for the presence of the transgene by a polymerase chain reaction genotyping assay. The number of transgenic animals typically varies from two to eight.^[7]

Chimeric mouse production consists of injecting embryonic stem cells provided by the investigator into 150–175 blastocysts, representing three days of work. Thirty to fifty live mice are normally born from this number of injected blastocysts. Normally, the skin color of the mice from which the host blastocysts are derived is different from that of the strain used to produce the embryonic stem cells. Typically two to six mice will have skin and hair with greater than seventy percent ES cell contribution, indicating a good chance for embryonic stem cell contribution to the germline.^[7]

Syrian hamsters

Syrian hamsters are used to model the human medical conditions including various cancers, metabolic diseases, non-cancer respiratory diseases, cardiovascular diseases, infectious diseases, and general health concerns.^[8] In 2006-07, Syrian hamsters accounted for 19% of the total animal research participants in the United States.^[9]

Rats

Limitations

While mice, rats and other rodents are by far the most widely used animals in biomedical research, recent studies have highlighted their limitations.^[10] For example, the utility of the use of rodents in testing for sepsis,^[11] burns,^[11] inflammation,^[11] stroke,^[12][13] ALS,^[14][15][16] Alzheimer’s,^[17] diabetes,^[18][19] cancer,^{[20][21][22][23][24]} multiple scrosis,^[25] Parkinson’s disease^[25] and other illnesses has been called into question by a number of researchers. Regarding experiments on mice in particular, some researchers have complained that “years and billions of dollars have been wasted following false leads” as a result of a preoccupation with the use of these animals in studies.^[10]

An article in The Scientist notes, “The difficulties associated with using animal models for human disease result from the metabolic, anatomic, and cellular differences between humans and other creatures, but the problems go even deeper than that” including issues with the design and execution of the tests themselves.^[13]

For example, researchers have found that many rats and mice in laboratories are obese from excess food and minimal exercise which alters their physiology and drug metabolism.^[26] Many laboratory animals, including mice and rats, are chronically stressed which can also negatively affect research outcomes and the ability to accurately extrapolate findings to humans.^[27][28] Researchers have also noted that many studies involving mice, rats and other rodents are poorly designed, leading to questionable findings.^[13][15][16]

See also

• Animal Testing
• Animal model
• BALB/c
• C57BL/6
• Mouse models of colorectal and intestinal cancer
• Preclinical imaging
• Rat Park
• Testing cosmetics on animals
• Mouse models of breast cancer metastasis

Notes

External links

• Information about mouse models from HOPES:Huntington's Disease Outreach Project for Education at Stanford
• Animal Model of Disease from Animal Research Organization